[ROLE OF CASPASE-3 IN REGULATION OF THE CONTENT OF THE AMYLOID-DEGRADING NEUROPEPTIDASE NEPRILYSIN IN THE CORTEX OF RATS AFTER HYPOXIA].
Zh Evol Biokhim Fiziol
; 51(6): 427-30, 2015.
Article
en Ru
| MEDLINE
| ID: mdl-26983277
ABSTRACT
Analysis of the effect of a caspase-3 inhibitor on the content of the amyloid-degrading neuropeptidase neprilysin (NEP) in the cortex of rats subjected to prenatal hypoxia (7% O2, 3 h) on the 14-th day of the embryonic development (E14) was performed. It was found that rats subjected to prenatal hypoxia on days 20-30 after birth have an increased content and activity of caspase-3 with reduced levels of NEP and of the C-terminal fragment of the amyloid precursor protein (AICD) regulating NEP expression. In hypoxic animals 3 days after a single injection of a caspase inhibitor (i. v., Ac-DEVD-CHO, P20) the content of AICD and NEP was found to be increased up to the levels observed in control rats. The data obtained suggest that the increase of caspase-3 enzyme activity could affect NEP expression via proteolytic degradation of its transcription factor AICD. These data for the first time demonstrate the role of caspases in AICD-dependent regulation of NEP production in the brain of mammals under hypoxic conditions.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neprilisina
/
Regulación Enzimológica de la Expresión Génica
/
Hipoxia Encefálica
/
Corteza Cerebral
/
Caspasa 3
Límite:
Animals
Idioma:
Ru
Revista:
Zh Evol Biokhim Fiziol
Año:
2015
Tipo del documento:
Article