Structure-activity relationship of the peptide binding-motif mediating the BRCA2:RAD51 protein-protein interaction.
FEBS Lett
; 590(8): 1094-102, 2016 04.
Article
en En
| MEDLINE
| ID: mdl-26992456
ABSTRACT
RAD51 is a recombinase involved in the homologous recombination of double-strand breaks in DNA. RAD51 forms oligomers by binding to another molecule of RAD51 via an 'FxxA' motif, and the same recognition sequence is similarly utilised to bind BRCA2. We have tabulated the effects of mutation of this sequence, across a variety of experimental methods and from relevant mutations observed in the clinic. We use mutants of a tetrapeptide sequence to probe the binding interaction, using both isothermal titration calorimetry and X-ray crystallography. Where possible, comparison between our tetrapeptide mutational study and the previously reported mutations is made, discrepancies are discussed and the importance of secondary structure in interpreting alanine scanning and mutational data of this nature is considered.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Péptidos
/
Proteína BRCA2
/
Recombinasa Rad51
Límite:
Humans
Idioma:
En
Revista:
FEBS Lett
Año:
2016
Tipo del documento:
Article
País de afiliación:
Reino Unido