Newly identified allatostatin Bs and their receptor in the two-spotted cricket, Gryllus bimaculatus.
Peptides
; 80: 25-31, 2016 06.
Article
en En
| MEDLINE
| ID: mdl-27018343
ABSTRACT
A cDNA encoding allatostatin Bs (ASTBs) containing the W(X)6W motif was identified using a database generated by a next generation sequencer (NGS) in the two-spotted cricket, Gryllus bimaculatus. The contig sequence revealed the presence of five novel putative ASTBs (GbASTBs) in addition to GbASTBs previously identified in G. bimaculatus. MALDI-TOF MS analyses revealed the presence of these novel and previously identified GbASTBs with three missing GbASTBs. We also identified a cDNA encoding G. bimaculatus GbASTB receptor (GbASTBR) in the NGS data. Phylogenetic analysis demonstrated that this receptor was highly conserved with other insect ASTBRs, including the sex peptide receptor of Drosophila melanogaster. Calcium imaging analyses indicated that the GbASTBR heterologously expressed in HEK293 cells exhibited responses to all identified GbASTBs at a concentration range of 10(-10)-10(-5)M.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neuropéptidos
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Gryllidae
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Receptores de Neuropéptido
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Proteínas de Insectos
Límite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Peptides
Año:
2016
Tipo del documento:
Article
País de afiliación:
Japón