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Improvement and automation of a real-time PCR assay for vaginal fluids.
De Vittori, E; Giampaoli, S; Barni, F; Baldi, M; Berti, A; Ripani, L; Romano Spica, V.
Afiliación
  • De Vittori E; University of Rome "Foro Italico", Department of Movement, Human and Health Sciences, Public Health Unit, Piazza Lauro De Bosis, 6, 00135 Rome, Italy; Legal Genetics - forensic consulting, Via Po, 102, 00198 Rome, Italy.
  • Giampaoli S; University of Rome "Foro Italico", Department of Movement, Human and Health Sciences, Public Health Unit, Piazza Lauro De Bosis, 6, 00135 Rome, Italy.
  • Barni F; Carabinieri, Reparto Investigazioni Scientifiche di Roma, Viale di Tor di Quinto, 119, 00191 Rome, Italy.
  • Baldi M; Legal Genetics - forensic consulting, Via Po, 102, 00198 Rome, Italy.
  • Berti A; Carabinieri, Reparto Investigazioni Scientifiche di Roma, Viale di Tor di Quinto, 119, 00191 Rome, Italy.
  • Ripani L; Carabinieri, Reparto Investigazioni Scientifiche di Roma, Viale di Tor di Quinto, 119, 00191 Rome, Italy.
  • Romano Spica V; University of Rome "Foro Italico", Department of Movement, Human and Health Sciences, Public Health Unit, Piazza Lauro De Bosis, 6, 00135 Rome, Italy. Electronic address: vincenzo.romanospica@uniroma4.it.
Forensic Sci Int ; 262: 179-82, 2016 May.
Article en En | MEDLINE | ID: mdl-27022861
The identification of vaginal fluids is crucial in forensic science. Several molecular protocols based on PCR amplification of mfDNA (microflora DNA) specific for vaginal bacteria are now available. Unfortunately mfDNA extraction and PCR reactions require manual optimization of several steps. The aim of present study was the verification of a partial automatization of vaginal fluids identification through two instruments widely diffused in forensic laboratories: EZ1 Advanced robot and Rotor Gene Q 5Plex HRM. Moreover, taking advantage of 5-plex thermocycler technology, the ForFluid kit performances were improved by expanding the mfDNA characterization panel with a new bacterial target for vaginal fluids and with an internal positive control (IPC) to monitor PCR inhibition. Results underlined the feasibility of a semi-automated extraction of mfDNA using a BioRobot and demonstrated the analytical improvements of the kit.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Automatización / Vagina / ADN Bacteriano / Reacción en Cadena en Tiempo Real de la Polimerasa Límite: Adult / Aged / Female / Humans / Middle aged Idioma: En Revista: Forensic Sci Int Año: 2016 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Irlanda

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Automatización / Vagina / ADN Bacteriano / Reacción en Cadena en Tiempo Real de la Polimerasa Límite: Adult / Aged / Female / Humans / Middle aged Idioma: En Revista: Forensic Sci Int Año: 2016 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Irlanda