PROTECTIVE EFFECTS OF POTASSIUM TRANSPORT IN MITOCHONDRIA FROM RAT MYOMETRIUM UNDER ACTIVATION OF MITOCHONDRIAL PERMEABILITY TRANSITION PORE.
Ukr Biochem J
; 87(6): 86-94, 2015.
Article
en En
| MEDLINE
| ID: mdl-27025062
ABSTRACT
We demonstrated using PBFI K(+)-sensitive fluorescent probe an enhancement of both components of K(+)-cycle--the ATP-sensitive K(+)-uptake and quinine-sensitive K+/H(+)-exchange--under the Ca2+ induced opening of mitochondrial permeability transition pore (MPTP) in rat myometrium mitochondria. Addition of CaCl2 (100 µM to K(+)-free medium results in the enhancement of reactive oxygen species (ROS) production, which was eliminated by cyclosporine A. Addition of CaCl2 to K(+)-rich medium did not increase the rate of ROS production, but blocking of mitoK+(ATP)-channels with glybenclamide (10 mcM increased production of ROS. We conclude that K(+)-cycle exerts a protective influence in mitochondria from rat myometrium by regulation of matrix volume and rate of ROS production under the condition of Ca(2+)-induced MPTP.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Canales de Potasio
/
Antiportadores de Potasio-Hidrógeno
/
Proteínas de Transporte de Membrana Mitocondrial
/
Mitocondrias
Límite:
Animals
Idioma:
En
Revista:
Ukr Biochem J
Año:
2015
Tipo del documento:
Article