[TYPING OF LEPTOSPIRA SPP. STRAINS BASED ON 16S rRNA].
Zh Mikrobiol Epidemiol Immunobiol
; (1): 35-9, 2016.
Article
en Ru
| MEDLINE
| ID: mdl-27029114
ABSTRACT
AIM:
Comparative typing of Leptospira spp. strain collection based on analysis of 16S RNA fragment. MATERIALS ANDMETHODS:
2 pairs of primers were used for PCR, that jointly flank 1423b.p. sized fragment. Sequences of Leptospira spp. strain 16S rRNA, presented in the international database, were used for phylogenetic analysis.RESULTS:
A high similarity, including interspecies, of the 16S fragment in Leptospira spp. strains was shown independently of the source, serovar and serogroup. Heterogeneity of the primary matrix, spontaneous mutations of hotspots and erroneous nucleotide couplings, characteristic for 16S sequence of pathogenic Leptospira spp. strains, are discussed. Molecular-genetic characteristic of certain reference Leptospira spp. strains by 16S sequence is obtained.CONCLUSION:
Results of the studies give evidence on expedience of introduction into clinical practice of identification of Leptospira spp. by 16S sequence directly from the clinical material, that would allow to significantly reduce identification time, dismiss complex type-specific sera and other labor-intensive methods.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Filogenia
/
ADN Bacteriano
/
ARN Ribosómico 16S
/
Técnicas de Tipificación Bacteriana
/
Leptospira
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
Ru
Revista:
Zh Mikrobiol Epidemiol Immunobiol
Año:
2016
Tipo del documento:
Article