Reverse genetics in high throughput: rapid generation of complete negative strand RNA virus cDNA clones and recombinant viruses thereof.

Nolden, T; Pfaff, F; Nemitz, S; Freuling, C M; Höper, D; Müller, T; Finke, Stefan

Nolden, T; Pfaff, F; Nemitz, S; Freuling, C M; Höper, D; Müller, T; Finke, Stefan.

Afiliación

Nolden T; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Molecular Virology and Cell Biology, D-17493 Greifswald - Insel Riems, Germany.
Pfaff F; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Diagnostic Virology, D-17493 Greifswald - Insel Riems, Germany.
Nemitz S; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Molecular Virology and Cell Biology, D-17493 Greifswald - Insel Riems, Germany.
Freuling CM; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Molecular Virology and Cell Biology, D-17493 Greifswald - Insel Riems, Germany.
Höper D; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Diagnostic Virology, D-17493 Greifswald - Insel Riems, Germany.
Müller T; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Molecular Virology and Cell Biology, D-17493 Greifswald - Insel Riems, Germany.
Finke S; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Molecular Virology and Cell Biology, D-17493 Greifswald - Insel Riems, Germany.

Sci Rep ; 6: 23887, 2016 Apr 05.

Article en En | MEDLINE | ID: mdl-27046474

Asunto(s)

Genoma Viral; Virus ARN/genética; Virus de la Rabia/genética; Genética Inversa/métodos; Animales; Línea Celular; Clonación Molecular; ADN Complementario/metabolismo; Perros; Escherichia coli/metabolismo; Zorros; Genes Reporteros; Proteínas Fluorescentes Verdes/metabolismo; Cinética; Proteínas Luminiscentes/metabolismo; Ratones; Mutación; Oligonucleótidos/genética; Reacción en Cadena de la Polimerasa; Proteínas Recombinantes; Recombinación Genética; Replicación Viral; Proteína Fluorescente Roja

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus de la Rabia / Virus ARN / Genoma Viral / Genética Inversa Límite: Animals Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido

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