Mycothiol peroxidase MPx protects Corynebacterium glutamicum against acid stress by scavenging ROS.
Biotechnol Lett
; 38(7): 1221-8, 2016 Jul.
Article
en En
| MEDLINE
| ID: mdl-27053080
OBJECTIVES: To investigate mycothiol peroxidase (MPx) of Corynebacterium glutamicum that is a novel CysGPx family peroxidase using both the mycoredoxin and thioredoxin reducing systems as proton donors for peroxide detoxification and may be involved in the relief of acid stress. RESULTS: A Δmpx mutant exhibited significantly decreased resistance to acid stress and markedly increased accumulation of reactive oxygen species (ROS) and protein carbonylation levels in vivo. Over-expression of mpx increased the resistance of C. glutamicum to acid stress by reducing ROS accumulation. The stress-responsive extracytoplasmic function-sigma (ECF-σ) factor, SigH, mediated acid-induced expression of mpx in the wild-type under acid conditions, which in turn directly contributed to tolerance to acid stress. CONCLUSION: MPx is essential for combating acid stress by reducing intracellular ROS levels induced by acid stress in C. glutamicum, which adds a new dimension to the general physiological functions of CysGPx.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Peroxidasas
/
Glicopéptidos
/
Especies Reactivas de Oxígeno
/
Cisteína
/
Corynebacterium glutamicum
/
Inositol
Idioma:
En
Revista:
Biotechnol Lett
Año:
2016
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Países Bajos