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Mycothiol peroxidase MPx protects Corynebacterium glutamicum against acid stress by scavenging ROS.
Wang, Tietao; Gao, Fen; Kang, Yiwen; Zhao, Chao; Su, Tao; Li, Muhang; Si, Meiru; Shen, Xihui.
Afiliación
  • Wang T; State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.
  • Gao F; State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.
  • Kang Y; State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.
  • Zhao C; State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.
  • Su T; College of Life Sciences, Qufu Normal University, Qufu, 273165, Shandong, China.
  • Li M; State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.
  • Si M; College of Life Sciences, Qufu Normal University, Qufu, 273165, Shandong, China.
  • Shen X; State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China. xihuishen@nwsuaf.edu.cn.
Biotechnol Lett ; 38(7): 1221-8, 2016 Jul.
Article en En | MEDLINE | ID: mdl-27053080
OBJECTIVES: To investigate mycothiol peroxidase (MPx) of Corynebacterium glutamicum that is a novel CysGPx family peroxidase using both the mycoredoxin and thioredoxin reducing systems as proton donors for peroxide detoxification and may be involved in the relief of acid stress. RESULTS: A Δmpx mutant exhibited significantly decreased resistance to acid stress and markedly increased accumulation of reactive oxygen species (ROS) and protein carbonylation levels in vivo. Over-expression of mpx increased the resistance of C. glutamicum to acid stress by reducing ROS accumulation. The stress-responsive extracytoplasmic function-sigma (ECF-σ) factor, SigH, mediated acid-induced expression of mpx in the wild-type under acid conditions, which in turn directly contributed to tolerance to acid stress. CONCLUSION: MPx is essential for combating acid stress by reducing intracellular ROS levels induced by acid stress in C. glutamicum, which adds a new dimension to the general physiological functions of CysGPx.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Peroxidasas / Glicopéptidos / Especies Reactivas de Oxígeno / Cisteína / Corynebacterium glutamicum / Inositol Idioma: En Revista: Biotechnol Lett Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Peroxidasas / Glicopéptidos / Especies Reactivas de Oxígeno / Cisteína / Corynebacterium glutamicum / Inositol Idioma: En Revista: Biotechnol Lett Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos