Phytochemical screening and anti-inflammatory properties of Algerian Hertia cheirifolia methanol extract.
Pharm Biol
; 54(11): 2584-2590, 2016 Nov.
Article
en En
| MEDLINE
| ID: mdl-27159241
ABSTRACT
CONTEXT Hertia cheirifolia L. (Asteraceae) is traditionally used in Northern Africa to treat various inflammatory infections. However, few studies on this plant have been reported. OBJECTIVE:
The anti-inflammatory activity of methanol extract of H. cheirifolia leaves was investigated using different experimental models. MATERIALS ANDMETHODS:
Phytochemical analysis was performed to determine phenolic compounds. Acute toxicity of the extract (2000 mg/kg) was examined in Swiss albino mice for 14 days, before croton oil-induced ear oedema in mice, carrageenan-induced paw oedema in Swiss albino rats, cotton pellet-induced granuloma in rats and carrageenan-induced air pouch in mice were conducted. The IL-1ß and TNF-α release from concanavalin A-stimulated monocytes was measured by ELISA.RESULTS:
Methanol extract of H. cheirifolia is rich in polyphenols and flavonoids. Cinnamic acid and rutin represent the major constituents. Methanol extract up to 2000 mg/kg did not produce any toxic effects. Topical application of 2 mg/ear of the extract produced 78.7% of inhibition on ear swilling. Oral pre-treatment of rats with 200 and 400 mg/kg of the extract inhibited paw oedema by 70% and 89%, respectively. At 200 mg/kg, granuloma dry and wet weights were reduced by 41.85% and 61.72%, respectively. Moreover, the treatment with methanol extract at 1 mg/kg exerted 62.7% of inhibition on leucocytes migrated into the ear pouch. TNF-α and IL-1ß release was reduced by 69% and 78%, respectively, with 1 µg/mL of the extract.CONCLUSION:
Methanol extract of H. cheirifolia possesses a strong anti-inflammatory activity and may be considered an interesting source of effective anti-inflammatory compounds.Palabras clave
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Extractos Vegetales
/
Asteraceae
/
Antiinflamatorios
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Límite:
Animals
Idioma:
En
Revista:
Pharm Biol
Año:
2016
Tipo del documento:
Article
País de afiliación:
Argelia