'2A-Like' Signal Sequences Mediating Translational Recoding: A Novel Form of Dual Protein Targeting.
Traffic
; 17(8): 923-39, 2016 08.
Article
en En
| MEDLINE
| ID: mdl-27161495
ABSTRACT
We report the initial characterization of an N-terminal oligopeptide '2A-like' sequence that is able to function both as a signal sequence and as a translational recoding element. Owing to this translational recoding activity, two forms of nascent polypeptide are synthesized (i) when 2A-mediated translational recoding has not occurred the nascent polypeptide is fused to the 2A-like N-terminal signal sequence and the fusion translation product is targeted to the exocytic pathway, and, (ii) a translation product where 2A-mediated translational recoding has occurred the 2A-like signal sequence is synthesized as a separate translation product and, therefore, the nascent (downstream) polypeptide lacks the 2A-like signal sequence and is localized to the cytoplasm. This type of dual-functional signal sequence results, therefore, in the partitioning of the translation products between the two sub-cellular sites and represents a newly described form of dual protein targeting.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ribosomas
/
Biosíntesis de Proteínas
/
Señales de Clasificación de Proteína
/
Transporte de Proteínas
Límite:
Humans
Idioma:
En
Revista:
Traffic
Asunto de la revista:
FISIOLOGIA
Año:
2016
Tipo del documento:
Article
País de afiliación:
Reino Unido