Expression and function of microRNA-497 in human osteosarcoma.

ABSTRACT

The expression and function of microRNA-497 (miR-497) has previously been reported in various types of human cancer; however, miR-497 has not previously been investigated in human osteosarcoma (OS). In the present study, the expression levels of miR­497 were analyzed by reverse transcription­quantitative polymerase chain reaction (RT­qPCR) in OS tissues and cell lines. In addition, post-transfection with miR­497, RT­qPCR, cell proliferation, migration and invasion assays, western blot analysis, and luciferase assays were performed in OS cell lines. The results of the present study demonstrated that miR­497 was downregulated in OS tissues and cells compared with normal controls. Furthermore, upregulation of miR­497 inhibited cell proliferation, migration and invasion in osteosarcoma cell lines compared with the negative control group. In addition, the present study demonstrated that miR­497 may function by directly targeting insulin­like growth factor 1 receptor in OS cells. These findings indicated that miR­497 may be useful as a therapeutic target for the treatment of OS.