Generation of a new Gateway-compatible inducible lentiviral vector platform allowing easy derivation of co-transduced cells.
Biotechniques
; 60(5): 252-9, 2016.
Article
en En
| MEDLINE
| ID: mdl-27177818
ABSTRACT
In contrast to most common gene delivery techniques, lentiviral vectors allow targeting of almost any mammalian cell type, even non-dividing cells, and they stably integrate in the genome. Therefore, these vectors are a very powerful tool for biomedical research. Here we report the generation of a versatile new set of 22 lentiviral vectors with broad applicability in multiple research areas. In contrast to previous systems, our platform provides a choice between constitutive and/or conditional expression and six different C-terminal fusions. Furthermore, two compatible selection markers enable the easy derivation of stable cell lines co-expressing differently tagged transgenes in a constitutive or inducible manner. We show that all of the vector features are functional and that they contribute to transgene overexpression in proof-of-principle experiments.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Recombinantes
/
Ingeniería Genética
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Lentivirus
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Transgenes
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Vectores Genéticos
Idioma:
En
Revista:
Biotechniques
Año:
2016
Tipo del documento:
Article
País de afiliación:
Bélgica
Pais de publicación:
ENGLAND
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ESCOCIA
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GB
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GREAT BRITAIN
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INGLATERRA
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REINO UNIDO
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SCOTLAND
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UK
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UNITED KINGDOM