Enhancement of DNA double-strand break induction and cell killing by K-shell absorption of phosphorus in human cell lines.
Int J Radiat Biol
; 92(11): 724-732, 2016 11.
Article
en En
| MEDLINE
| ID: mdl-27185241
ABSTRACT
PURPOSE:
To investigate an enhancement of DNA double-strand break (DSB) induction and cell killing effect by K-shell ionization of phosphorus atoms and Auger electrons on human cell lines. MATERIALS ANDMETHODS:
Induction of DSB, DNA damage responses, cell cycle distributions, and cell killing effects were investigated after exposures of the cells with monochromatic synchrotron radiation soft X-rays of 2153 and 2147 eV, which were the resonance peak and off peak, respectively, of the K-shell photoabsorption of phosphorus.RESULTS:
Higher biological effects in the cells irradiated with soft X-rays at 2153 eV than at 2147 eV were observed in (i) the efficiency of 53BP1/γ-H2AX co-localized foci formation per dose and residual number of foci, (ii) prolonged phosphorylation levels of DSB repair and/or cell cycle checkpoint related proteins and G2 arrest, (iii) the cell killing effects at the 10% survival level of normal human fibroblasts, HeLa cells, and human glioblastoma M059K cells (1.2-1.5 times higher) and that of human ataxia telangiectasia mutated (ATM)-defective cells and glioblastoma DNA-dependent protein kinase catalytic subunit (DNA-PKcs)-defective cells (1.2 times).CONCLUSION:
The yield of DSB and partly less-reparable complex DNA damage induction in human cells was enhanced by K-shell photoabsorption of phosphorus and low-energy Auger electrons.Palabras clave
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fósforo
/
Daño del ADN
/
Apoptosis
/
Neoplasias Experimentales
Límite:
Humans
Idioma:
En
Revista:
Int J Radiat Biol
Asunto de la revista:
RADIOLOGIA
Año:
2016
Tipo del documento:
Article