ALG-2 interacting protein-X (Alix) is essential for clathrin-independent endocytosis and signaling.
Sci Rep
; 6: 26986, 2016 05 31.
Article
en En
| MEDLINE
| ID: mdl-27244115
ABSTRACT
The molecular mechanisms and the biological functions of clathrin independent endocytosis (CIE) remain largely elusive. Alix (ALG-2 interacting protein X), has been assigned roles in membrane deformation and fission both in endosomes and at the plasma membrane. Using Alix ko cells, we show for the first time that Alix regulates fluid phase endocytosis and internalization of cargoes entering cells via CIE, but has no apparent effect on clathrin mediated endocytosis or downstream endosomal trafficking. We show that Alix acts with endophilin-A to promote CIE of cholera toxin and to regulate cell migration. We also found that Alix is required for fast endocytosis and downstream signaling of the interleukin-2 receptor giving a first indication that CIE is necessary for activation of at least some surface receptors. In addition to characterizing a new function for Alix, our results highlight Alix ko cells as a unique tool to unravel the biological consequences of CIE.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Endosomas
/
Proteínas de Unión al Calcio
/
Aciltransferasas
/
Receptores de Interleucina-2
/
Proteínas de Ciclo Celular
/
Endocitosis
/
Complejos de Clasificación Endosomal Requeridos para el Transporte
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Sci Rep
Año:
2016
Tipo del documento:
Article
País de afiliación:
Francia