Assay of FAAH Activity.

Bari, Monica; Feole, Monica; Maccarrone, Mauro

Bari, Monica; Feole, Monica; Maccarrone, Mauro.

Afiliación

Bari M; Department of Experimental Medicine and Surgery, Tor Vergata University of Rome, Rome, Italy. Bari@med.uniroma2.it.
Feole M; Center of Integrated Research, Campus Bio-Medico University of Rome, Rome, Italy.
Maccarrone M; Center of Integrated Research, Campus Bio-Medico University of Rome, Rome, Italy.

Methods Mol Biol ; 1412: 131-6, 2016.

Article en En | MEDLINE | ID: mdl-27245899

ABSTRACT

ABSTRACT

Fatty acid amide hydrolase (FAAH) is an intracellular enzyme responsible for the hydrolysis of endogenous anandamide (AEA), a reaction that terminates the biological effects of this lipid mediator. The final products of this reaction are arachidonic acid and ethanolamine. In the method described herein, FAAH activity is measured through the use of a radioactive substrate by quantification of reaction products, that is, [(14)C]-ethanolamine from [(14)C-ethanolamine]-AEA.

Asunto(s)

Amidohidrolasas/metabolismo; Pruebas de Enzimas; Animales; Ácido Araquidónico/metabolismo; Ácidos Araquidónicos/metabolismo; Encéfalo/enzimología; Endocannabinoides/metabolismo; Activación Enzimática; Etanolamina/metabolismo; Metabolismo de los Lípidos; Ratones; Alcamidas Poliinsaturadas/metabolismo

Palabras clave

AEA; Arachidonic acid; Bioactive lipids; Endocannabinoids; Enzyme; Ethanolamine; FAAH

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Enzimas / Amidohidrolasas Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2016 Tipo del documento: Article País de afiliación: Italia

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