The antimicrobial potential of stevia in an in vitro microbial caries model.
Am J Dent
; 29(2): 87-92, 2016 Apr.
Article
en En
| MEDLINE
| ID: mdl-27295866
ABSTRACT
PURPOSE:
To determine the effect of stevia on caries development when incorporated into a cariogenic diet in a controlled microbial caries model.METHODS:
56 bovine tooth specimens (4 x 4 mm) were divided into four groups, each secured in a caries-forming vessel. All vessels were placed on an electric stirrer inside a 37°C incubator. The specimens were inoculated with Streptococcus mutans, and exposed for 4 days to circulating cycles of tryptic soy broth supplemented with 5% sucrose-TSBS (three x/day), and a mineral wash solution. Between TSBS cycles (three x/day), each group received one of four experimental solutions phosphate buffer (PBS-negative control), 0.5% stevia solution, 5% stevia solution, or 5% xylitol solution. Development of caries lesions was analyzed using enamel surface hardness. Difference in Vickers Hardness between pre and post-treatment was calculated to determine caries development. Plaque was dislodged from six specimens per group, and the CFU/ml calculated. Data were analyzed using ANOVA at 95% confidence level, and individual group differences calculated using Tukey's test.RESULTS:
5% xylitol resulted in significantly less plaque at the end of the study compared to PBS and 5% stevia, but not significantly different than 0.5% stevia. 5% stevia had significantly softer lesions than the other groups, while there was no significant difference in hardness scores between 5% xylitol, 0.5% stevia and PBS.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Stevia
/
Caries Dental
/
Antiinfecciosos
/
Modelos Biológicos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Am J Dent
Asunto de la revista:
ODONTOLOGIA
Año:
2016
Tipo del documento:
Article