Sirtuin 1-dependent resveratrol cytotoxicity and pro-differentiation activity on breast cancer cells.
Arch Toxicol
; 91(3): 1261-1278, 2017 Mar.
Article
en En
| MEDLINE
| ID: mdl-27358235
ABSTRACT
Sirtuins regulate several processes associated with tumor development. Resveratrol was shown to stimulate sirtuin 1 and 3 (SIRT1/3) activities and to result in cytotoxicity for some tumor types. The relationship between modulation of sirtuin activities, cellular metabolic remodeling and resveratrol cytotoxicity mechanism on breast cancer cells is still an open question. Here, we evaluated whether sirtuin 1 and 3 are involved in resveratrol toxicity and whether resveratrol leads to a metabolic remodeling and cell differentiation. Results using the Extracellular Flux Analyzer indicated that resveratrol inhibits mitochondrial respiration in breast cancer cells. We also demonstrated here for the first time that resveratrol cytotoxic effects on breast cancer cells were modulated by SIRT1 and also involved mitochondrial complex I inhibition. Importantly, we also demonstrated that resveratrol reduced the pool of breast cancer cells with stemness markers through a SIRT1-dependent mechanism. Our data highlights the role of SIRT1 in regulating resveratrol induced differentiation and/or toxicity in breast cancer cells.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Estilbenos
/
Neoplasias de la Mama
/
Sirtuina 1
Límite:
Animals
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Female
/
Humans
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Male
Idioma:
En
Revista:
Arch Toxicol
Año:
2017
Tipo del documento:
Article
País de afiliación:
Portugal