Visualization and analysis of gene expression in tissue sections by spatial transcriptomics.
Science
; 353(6294): 78-82, 2016 Jul 01.
Article
en En
| MEDLINE
| ID: mdl-27365449
ABSTRACT
Analysis of the pattern of proteins or messengerRNAs (mRNAs) in histological tissue sections is a cornerstone in biomedical research and diagnostics. This typically involves the visualization of a few proteins or expressed genes at a time. We have devised a strategy, which we call "spatial transcriptomics," that allows visualization and quantitative analysis of the transcriptome with spatial resolution in individual tissue sections. By positioning histological sections on arrayed reverse transcription primers with unique positional barcodes, we demonstrate high-quality RNA-sequencing data with maintained two-dimensional positional information from the mouse brain and human breast cancer. Spatial transcriptomics provides quantitative gene expression data and visualization of the distribution of mRNAs within tissue sections and enables novel types of bioinformatics analyses, valuable in research and diagnostics.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Análisis de Secuencia de ARN
/
Perfilación de la Expresión Génica
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Transcriptoma
Límite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Science
Año:
2016
Tipo del documento:
Article
País de afiliación:
Suecia