Quantification of Rac1 and Rac1b in serum of non small cell lung cancer by label free real time assay.
Clin Chim Acta
; 460: 231-5, 2016 Sep 01.
Article
en En
| MEDLINE
| ID: mdl-27425849
ABSTRACT
BACKGROUND:
Rac proteins play a major role in tumorogenesis. We quantified Rac1 and Rac1b in serum of non small cell lung cancer (NSCLC) patients.METHODS:
The blood of 77 NSCLC patients and 52 healthy controls were collected and quantified the concentration of Rac1 and Rac1b mainly by surface plasmon resonance and it was verify by Western blot analysis.RESULTS:
Rac1 and Rac1b were found to be significantly over expressed in serum of NSCLC patients compare to healthy controls. The level of Rac proteins were found to be increased in all stages of cancer. Despite the low survival rate, we managed to collect serum sample of the 18 follow up patients after the therapy, where 11 patients' of CR+PR group showed down regulation of the Rac protein after chemotherapy and unfortunately 80% patients died during the study period.CONCLUSION:
The high specificity and sensitivity obtained from ROC analysis for Rac1 and Rac1b envisaged it to be used as a serum diagnostic marker in the early stage of cancer.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Carcinoma de Pulmón de Células no Pequeñas
/
Proteína de Unión al GTP rac1
Tipo de estudio:
Diagnostic_studies
/
Observational_studies
Límite:
Humans
Idioma:
En
Revista:
Clin Chim Acta
Año:
2016
Tipo del documento:
Article
País de afiliación:
India