Experimental strategies for in vivo13C NMR spectroscopy.
Anal Biochem
; 529: 216-228, 2017 07 15.
Article
en En
| MEDLINE
| ID: mdl-27515993
ABSTRACT
In vivo carbon-13 (13C) MRS opens unique insights into the metabolism of intact organisms, and has led to major advancements in the understanding of cellular metabolism under normal and pathological conditions in various organs such as skeletal muscles, the heart, the liver and the brain. However, the technique comes at the expense of significant experimental difficulties. In this review we focus on the experimental aspects of non-hyperpolarized 13C MRS in vivo. Some of the enrichment strategies which have been proposed so far are described; the various MRS acquisition paradigms to measure 13C labeling are then presented. Finally, practical aspects of 13C spectral quantification are discussed.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Isótopos de Carbono
/
Imagen por Resonancia Magnética
/
Espectroscopía de Resonancia Magnética
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Anal Biochem
Año:
2017
Tipo del documento:
Article