[Role of epithelial sodium channel in rat osteoclast differentiation and bone resorption].
Nan Fang Yi Ke Da Xue Xue Bao
; 36(8): 1148-52, 2016 Aug 20.
Article
en Zh
| MEDLINE
| ID: mdl-27578589
ABSTRACT
OBJECTIVE:
To explore the role of epithelial sodium channel (ENaC) in regulating the functional activity of osteoclasts.METHODS:
Multinucleated osteoclasts were obtained by inducing the differentiation of rat bone marrow cells with macrophage colony-stimulating factor (M-CSF) and RANKL. The osteoclasts were exposed to different concentrations of the ENaC inhibitor amiloride, and the expression of ENaC on osteoclasts was examined using immunofluorescence technique. The osteoclasts were identified with tartrate-resistant acid phosphatase (TRAP) staining, and the positive cells were incubated with fresh bovine femoral bone slices and the number of bone absorption pits was counted by computer-aided image processing. RT-PCR was performed to analyze the expression of cathepsin K in the osteoclasts.RESULTS:
s Exposure to different concentrations of amiloride significantly inhibited the expression of ENaC and reduced the number of TRAP-positive osteoclasts. Exposure of the osteoclasts to amiloride also reduced the number of bone resorption pits on bone slices and the expression of osteoclast-specific gene cathepsin K.CONCLUSION:
s ENaC may participate in the regulation of osteoclast differentiation and bone resorption, suggesting its role in functional regulation of the osteoclasts and a possibly new signaling pathway related with ENaC regulation for modulating bone metabolism.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Osteoclastos
/
Resorción Ósea
/
Canales Epiteliales de Sodio
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Nan Fang Yi Ke Da Xue Xue Bao
Año:
2016
Tipo del documento:
Article