1.65â
Å resolution structure of the AraC-family transcriptional activator ToxT from Vibrio cholerae.
Acta Crystallogr F Struct Biol Commun
; 72(Pt 9): 726-31, 2016 09.
Article
en En
| MEDLINE
| ID: mdl-27599865
ABSTRACT
ToxT is an AraC-family transcriptional activator protein that controls the expression of key virulence factors in Vibrio cholerae, the causative agent of cholera. ToxT directly activates the expression of the genes that encode the toxin-coregulated pilus and cholera toxin, and also positively auto-regulates its own expression from the tcp promoter. The crystal structure of ToxT has previously been solved at 1.9â
Å resolution (PDB entry 3gbg). In this study, a crystal structure of ToxT at 1.65â
Å resolution with a similar overall structure to the previously determined structure is reported. However, there are distinct differences between the two structures, particularly in the region that extends from Asp101 to Glu110. This region, which can influence ToxT activity but was disordered in the previous structure, can be traced entirely in the current structure.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Factores de Transcripción
/
Vibrio cholerae
/
Proteínas Recombinantes de Fusión
/
Ácido Aspártico
/
Ácido Glutámico
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Acta Crystallogr F Struct Biol Commun
Año:
2016
Tipo del documento:
Article
País de afiliación:
Estados Unidos