Significant role for IRF3 in both T cell and APC effector functions during T cell responses.
Cell Immunol
; 310: 141-149, 2016 12.
Article
en En
| MEDLINE
| ID: mdl-27641636
ABSTRACT
Interferon Regulatory Factor (IRF)3 is a crucial transcription factor during innate immune responses. Here we show IRF3 also has a role in adaptive T cell immune responses. Expression of IFN-γ, IL-17, and Granzyme B (GrB) during in vitro T cell responses was impaired when either dendritic cells (DCs) or T cells were derived from IRF3KO mice. Unexpectedly, IRF3-dependent NK-activating molecule (INAM), which is an NK cell activating factor of the DC innate immune response, was induced during the T cell response. Additionally, supernatants from responding T cells induced ISG54 in the RAW264.7 macrophage cell line in an IRF3 dependent manner. Moreover, addition of anti-IFN-γ prevented supernatant induction of ISG54 and recombinant IFN-γ stimulated ISG54 expression. Thus, IRF3 in APCs and T cells is required for optimal T-cell effector function and the ability of T cells to influence innate immune function of APCs.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Dendríticas
/
Linfocitos T
/
Factor 3 Regulador del Interferón
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Macrófagos
/
Células Presentadoras de Antígenos
Límite:
Animals
Idioma:
En
Revista:
Cell Immunol
Año:
2016
Tipo del documento:
Article
País de afiliación:
Estados Unidos