Plasma-Treated Microplates with Enhanced Protein Recoveries and Minimized Extractables.
SLAS Technol
; 22(1): 98-105, 2017 02.
Article
en En
| MEDLINE
| ID: mdl-27651466
ABSTRACT
SiO2 Medical Products, Inc. (SiO) has developed a proprietary technology that greatly enhances protein recoveries and reduces extractables from commercial microplates used for bioanalytical assays and storage of biologics. SiO technology is based on plasma treatment that chemically modifies the surface of polypropylene with predominantly hydrogen-bond-acceptor uncharged polar groups. The resultant surface resists nonspecific protein adsorption over a wide range of protein concentrations, thereby eliminating the need to passivate (and hence potentially contaminate) the microplates with blocking proteins. High shelf-life stability and cleanliness of the plasma-treated microplates have been demonstrated using five different proteins for two common microplate formats. The protein recovery performance of plasma-treated microplates is found to be higher compared with commercial low-protein-binding microplates.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plasma
/
Polipropilenos
/
Propiedades de Superficie
/
Proteínas
/
Ciencia del Laboratorio Clínico
/
Materiales Biocompatibles Revestidos
/
Equipos y Suministros
Límite:
Animals
/
Humans
Idioma:
En
Revista:
SLAS Technol
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos