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Comparative evaluation of two Rickettsia typhi-specific quantitative real-time PCRs for research and diagnostic purposes.
Papp, Stefanie; Rauch, Jessica; Kuehl, Svenja; Richardt, Ulricke; Keller, Christian; Osterloh, Anke.
Afiliación
  • Papp S; Department of Immunology, Bernhard Nocht Institute for Tropical Medicine, 20359, Hamburg, Germany.
  • Rauch J; Department of Immunology, Bernhard Nocht Institute for Tropical Medicine, 20359, Hamburg, Germany.
  • Kuehl S; Department of Immunology, Bernhard Nocht Institute for Tropical Medicine, 20359, Hamburg, Germany.
  • Richardt U; Department of Immunology, Bernhard Nocht Institute for Tropical Medicine, 20359, Hamburg, Germany.
  • Keller C; Institute for Virology, University Medical Center Gießen and Marburg, 35032, Marburg, Germany.
  • Osterloh A; Department of Immunology, Bernhard Nocht Institute for Tropical Medicine, 20359, Hamburg, Germany. osterloh@bni-hamburg.de.
Med Microbiol Immunol ; 206(1): 41-51, 2017 Feb.
Article en En | MEDLINE | ID: mdl-27696011
Rickettsioses are caused by intracellular bacteria of the family of Rickettsiaceae. Rickettsia (R.) typhi is the causative agent of endemic typhus. The disease occurs worldwide and is one of the most prevalent rickettsioses. Rickettsial diseases, however, are generally underdiagnosed which is mainly due to the lack of sensitive and specific methods. In addition, methods for quantitative detection of the bacteria for research purposes are rare. We established two qPCRs for the detection of R. typhi by amplification of the outer membrane protein B (ompB) and parvulin-type PPIase (prsA) genes. Both qPCRs are specific and exclusively recognize R. typhi but no other rickettsiae including the closest relative, R. prowazekii. The prsA-based qPCR revealed to be much more sensitive than the amplification of ompB and provided highly reproducible results in the detection of R. typhi in organs of infected mice. Furthermore, as a nested PCR the prsA qPCR was applicable for the detection of R. typhi in human blood samples. Collectively, the prsA-based qPCR represents a reliable method for the quantitative detection of R. typhi for research purposes and is a promising candidate for differential diagnosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rickettsia typhi / Tifus Endémico Transmitido por Pulgas / Técnicas de Diagnóstico Molecular / Reacción en Cadena en Tiempo Real de la Polimerasa Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Animals / Humans Idioma: En Revista: Med Microbiol Immunol Año: 2017 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rickettsia typhi / Tifus Endémico Transmitido por Pulgas / Técnicas de Diagnóstico Molecular / Reacción en Cadena en Tiempo Real de la Polimerasa Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Animals / Humans Idioma: En Revista: Med Microbiol Immunol Año: 2017 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania