Galleria mellonella hemocytes: A novel phagocytic assay for Leishmania (Viannia) braziliensis.
J Microbiol Methods
; 131: 45-50, 2016 12.
Article
en En
| MEDLINE
| ID: mdl-27713019
ABSTRACT
Galleria mellonella is an excellent invertebrate model for the study of diseases that involve interactions with cells from the innate immune system, since they have an innate immune system capable of recognizing the pathogens. Here we present for the first time, an alternative model for an in vitro phagocytic assay using hemocytes of G. mellonella larvae to study infection by Leishmania (Viannia) braziliensis. We showed that the insect phagocytic cells were able to engulf promastigotes. Furthermore, this infective form differentiated into the amastigote form inside those cells. However, the cells in this model seem resistant to the parasite, since amastigotes were depleted after 24h and NO levels were maintained after infection. Our model opens an avenue of possibilities for new investigations regarding other Leishmania species, mechanisms of invasion and evasion, receptors involved, release of signaling molecules and, above all, it is a novel infection model using invertebrate animals.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fagocitos
/
Leishmania braziliensis
/
Leishmaniasis Mucocutánea
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Modelos Animales de Enfermedad
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Hemocitos
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Larva
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Lepidópteros
Límite:
Animals
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
J Microbiol Methods
Año:
2016
Tipo del documento:
Article
País de afiliación:
Brasil