Your browser doesn't support javascript.
loading
SPR-based assays enable the full functional analysis of bispecific molecules.
Meschendoerfer, W; Gassner, C; Lipsmeier, F; Regula, J T; Moelleken, J.
Afiliación
  • Meschendoerfer W; Large Molecule Research, Pharma Research and Early Development, Roche Innovation Center Munich, Germany. Electronic address: wolfgang.meschendoerfer@roche.com.
  • Gassner C; Large Molecule Research, Pharma Research and Early Development, Roche Innovation Center Munich, Germany.
  • Lipsmeier F; pRED Informatics, Pharma Research & Early Development, Roche Innovation Center Basel, Switzerland.
  • Regula JT; Large Molecule Research, Pharma Research and Early Development, Roche Innovation Center Munich, Germany.
  • Moelleken J; Large Molecule Research, Pharma Research and Early Development, Roche Innovation Center Munich, Germany.
J Pharm Biomed Anal ; 132: 141-147, 2017 Jan 05.
Article en En | MEDLINE | ID: mdl-27721070
The increasing complexity of novel biotherapeutics such as bispecific antibodies or fusion proteins raises new challenges for functional characterization. When compared to standard antibodies, two individual interactions and the inter-dependency of binding events need to be considered for bispecific antibodies. We have previously described an SPR-based assay setup, which enables us to assess the binding activity of a bivalent-bispecific molecule to both targets simultaneously and - in addition to one individual target - in a single setup. However, there might be some pitfalls when applying the bridging assay, e.g. change of antigen activity upon immobilization. Therefore, we have developed an alternative SPR-based assay principle, which allows the individual assessment of both targets in solution. Comparison of data between the assays showed that simultaneous binding can be calculated based on both individual readouts, and revealed a good correlation. Hence, both SPR-based assay principles allow a "full" functional analysis of a bispecific CrossMab in only one assay. The assay principles can be qualified and enable an efficient drug development.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bioensayo / Resonancia por Plasmón de Superficie Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: J Pharm Biomed Anal Año: 2017 Tipo del documento: Article Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bioensayo / Resonancia por Plasmón de Superficie Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: J Pharm Biomed Anal Año: 2017 Tipo del documento: Article Pais de publicación: Reino Unido