Micro-C XL: assaying chromosome conformation from the nucleosome to the entire genome.
Nat Methods
; 13(12): 1009-1011, 2016 Dec.
Article
en En
| MEDLINE
| ID: mdl-27723753
ABSTRACT
We present Micro-C XL, an improved method for analysis of chromosome folding at mononucleosome resolution. Using long crosslinkers and isolation of insoluble chromatin, Micro-C XL increases signal-to-noise ratio. Micro-C XL maps of budding and fission yeast genomes capture both short-range chromosome fiber features such as chromosomally interacting domains and higher order features such as centromere clustering. Micro-C XL provides a single assay to interrogate chromosome folding at length scales from the nucleosome to the full genome.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Schizosaccharomyces
/
Nucleosomas
/
Cromosomas Fúngicos
/
Mapeo Cromosómico
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2016
Tipo del documento:
Article
País de afiliación:
Estados Unidos