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TSPAN8 Expression Distinguishes Spermatogonial Stem Cells in the Prepubertal Mouse Testis.
Mutoji, Kazadi; Singh, Anukriti; Nguyen, Thu; Gildersleeve, Heidi; Kaucher, Amy V; Oatley, Melissa J; Oatley, Jon M; Velte, Ellen K; Geyer, Christopher B; Cheng, Keren; McCarrey, John R; Hermann, Brian P.
Afiliación
  • Mutoji K; Department of Biology, University of Texas at San Antonio, San Antonio, Texas.
  • Singh A; Department of Biology, University of Texas at San Antonio, San Antonio, Texas.
  • Nguyen T; Department of Biology, University of Texas at San Antonio, San Antonio, Texas.
  • Gildersleeve H; Department of Biology, University of Texas at San Antonio, San Antonio, Texas.
  • Kaucher AV; Genomics Core Facility, University of Texas at San Antonio, San Antonio, Texas.
  • Oatley MJ; Center for Reproductive Biology, School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, Washington.
  • Oatley JM; Center for Reproductive Biology, School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, Washington.
  • Velte EK; Center for Reproductive Biology, School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, Washington.
  • Geyer CB; Department of Anatomy and Cell Biology and East Carolina Diabetes and Obesity Institute, Brody School of Medicine, East Carolina University, Greenville, North Carolina.
  • Cheng K; Department of Anatomy and Cell Biology and East Carolina Diabetes and Obesity Institute, Brody School of Medicine, East Carolina University, Greenville, North Carolina.
  • McCarrey JR; Department of Biology, University of Texas at San Antonio, San Antonio, Texas.
  • Hermann BP; Department of Biology, University of Texas at San Antonio, San Antonio, Texas.
Biol Reprod ; 95(6): 117, 2016 12.
Article en En | MEDLINE | ID: mdl-27733379
Precise separation of spermatogonial stem cells (SSCs) from progenitor spermatogonia that lack stem cell activity and are committed to differentiation remains a challenge. To distinguish between these spermatogonial subtypes, we identified genes that exhibited bimodal mRNA levels at the single-cell level among undifferentiated spermatogonia from Postnatal Day 6 mouse testes, including Tspan8, Epha2, and Pvr, each of which encode cell surface proteins useful for cell selection. Transplantation studies provided definitive evidence that a TSPAN8-high subpopulation is enriched for SSCs. RNA-seq analyses identified genes differentially expressed between TSPAN8-high and -low subpopulations that clustered into multiple biological pathways potentially involved in SSC renewal or differentiation, respectively. Methyl-seq analysis identified hypomethylated domains in the promoters of these genes in both subpopulations that colocalized with peaks of histone modifications defined by ChIP-seq analysis. Taken together, these results demonstrate functional heterogeneity among mouse undifferentiated spermatogonia and point to key biological characteristics that distinguish SSCs from progenitor spermatogonia.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Testículo / Tetraspaninas / Células Madre Germinales Adultas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Biol Reprod Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Testículo / Tetraspaninas / Células Madre Germinales Adultas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Biol Reprod Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos