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Genome resources for climate-resilient cowpea, an essential crop for food security.
Muñoz-Amatriaín, María; Mirebrahim, Hamid; Xu, Pei; Wanamaker, Steve I; Luo, MingCheng; Alhakami, Hind; Alpert, Matthew; Atokple, Ibrahim; Batieno, Benoit J; Boukar, Ousmane; Bozdag, Serdar; Cisse, Ndiaga; Drabo, Issa; Ehlers, Jeffrey D; Farmer, Andrew; Fatokun, Christian; Gu, Yong Q; Guo, Yi-Ning; Huynh, Bao-Lam; Jackson, Scott A; Kusi, Francis; Lawley, Cynthia T; Lucas, Mitchell R; Ma, Yaqin; Timko, Michael P; Wu, Jiajie; You, Frank; Barkley, Noelle A; Roberts, Philip A; Lonardi, Stefano; Close, Timothy J.
Afiliación
  • Muñoz-Amatriaín M; Department of Botany and Plant Sciences, University of California, Riverside, CA, USA.
  • Mirebrahim H; Department of Computer Science and Engineering, University of California, Riverside, CA, USA.
  • Xu P; Institute of Vegetables, Zhejiang Academy of Agricultural Sciences (ZAAS), Hangzhou, 310021, China.
  • Wanamaker SI; Department of Botany and Plant Sciences, University of California, Riverside, CA, USA.
  • Luo M; Department of Plant Sciences, University of California, Davis, CA, USA.
  • Alhakami H; Department of Computer Science and Engineering, University of California, Riverside, CA, USA.
  • Alpert M; Department of Computer Science and Engineering, University of California, Riverside, CA, USA.
  • Atokple I; Council for Scientific and Industrial Research, Savanna Agricultural Research Institute, Tamale, Ghana.
  • Batieno BJ; Institut de l'Environnement et de Recherches Agricoles, Saria, Burkina Faso.
  • Boukar O; International Institute of Tropical Agriculture, Kano, Nigeria.
  • Bozdag S; Department of Computer Science and Engineering, University of California, Riverside, CA, USA.
  • Cisse N; Department of Mathematics, Statistics and Computer Science, Marquette University, Milwaukee, WI, USA.
  • Drabo I; Institut Sénégalais de Recherches Agricoles, Thiès, Senegal.
  • Ehlers JD; Institut de l'Environnement et de Recherches Agricoles, Saria, Burkina Faso.
  • Farmer A; Department of Botany and Plant Sciences, University of California, Riverside, CA, USA.
  • Fatokun C; The Bill & Melinda Gates Foundation, Seattle, WA, USA.
  • Gu YQ; National Center for Genome Resources, Santa Fe, NM, USA.
  • Guo YN; International Institute of Tropical Agriculture, Ibadan, Nigeria.
  • Huynh BL; USDA-ARS Western Regional Research Center, Albany, CA, USA.
  • Jackson SA; Department of Botany and Plant Sciences, University of California, Riverside, CA, USA.
  • Kusi F; Department of Nematology, University of California, Riverside, CA, USA.
  • Lawley CT; Center for Applied Genetic Technologies, University of Georgia, Athens, GA, USA.
  • Lucas MR; Council for Scientific and Industrial Research, Savanna Agricultural Research Institute, Tamale, Ghana.
  • Ma Y; Illumina, Inc., San Francisco, CA, USA.
  • Timko MP; Department of Botany and Plant Sciences, University of California, Riverside, CA, USA.
  • Wu J; Department of Botany and Plant Sciences, University of California, Riverside, CA, USA.
  • You F; Department of Plant Sciences, University of California, Davis, CA, USA.
  • Barkley NA; Department of Biology, University of Virginia, Charlottesville, VA, USA.
  • Roberts PA; Department of Plant Sciences, University of California, Davis, CA, USA.
  • Lonardi S; Department of Plant Sciences, University of California, Davis, CA, USA.
  • Close TJ; Agriculture and Agri-Food Canada, Morden, MB, Canada.
Plant J ; 89(5): 1042-1054, 2017 Mar.
Article en En | MEDLINE | ID: mdl-27775877
ABSTRACT
Cowpea (Vigna unguiculata L. Walp.) is a legume crop that is resilient to hot and drought-prone climates, and a primary source of protein in sub-Saharan Africa and other parts of the developing world. However, genome resources for cowpea have lagged behind most other major crops. Here we describe foundational genome resources and their application to the analysis of germplasm currently in use in West African breeding programs. Resources developed from the African cultivar IT97K-499-35 include a whole-genome shotgun (WGS) assembly, a bacterial artificial chromosome (BAC) physical map, and assembled sequences from 4355 BACs. These resources and WGS sequences of an additional 36 diverse cowpea accessions supported the development of a genotyping assay for 51 128 SNPs, which was then applied to five bi-parental RIL populations to produce a consensus genetic map containing 37 372 SNPs. This genetic map enabled the anchoring of 100 Mb of WGS and 420 Mb of BAC sequences, an exploration of genetic diversity along each linkage group, and clarification of macrosynteny between cowpea and common bean. The SNP assay enabled a diversity analysis of materials from West African breeding programs. Two major subpopulations exist within those materials, one of which has significant parentage from South and East Africa and more diversity. There are genomic regions of high differentiation between subpopulations, one of which coincides with a cluster of nodulin genes. The new resources and knowledge help to define goals and accelerate the breeding of improved varieties to address food security issues related to limited-input small-holder farming and climate stress.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Productos Agrícolas / Vigna Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Productos Agrícolas / Vigna Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos