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Suitability of cholinesterase of polychaete Diopatra neapolitana as biomarker of exposure to pesticides: In vitro characterization.
Mennillo, Elvira; Casu, Valentina; Tardelli, Federica; De Marchi, Lucia; Freitas, Rosa; Pretti, Carlo.
Afiliación
  • Mennillo E; Department of Veterinary Sciences, University of Pisa, San Piero a Grado, PI 56122, Italy.
  • Casu V; Department of Veterinary Sciences, University of Pisa, San Piero a Grado, PI 56122, Italy.
  • Tardelli F; Department of Veterinary Sciences, University of Pisa, San Piero a Grado, PI 56122, Italy.
  • De Marchi L; Departamento de Biologia & CESAM, University of Aveiro 3810-193, Portugal.
  • Freitas R; Departamento de Biologia & CESAM, University of Aveiro 3810-193, Portugal.
  • Pretti C; Department of Veterinary Sciences, University of Pisa, San Piero a Grado, PI 56122, Italy; Interuniversitary Center of Marine Biology (CIBM) "G. Bacci", Leghorn 57128, Italy. Electronic address: carlo.pretti@unipi.it.
Article en En | MEDLINE | ID: mdl-27777085
Cholinesterases of Diopatra neapolitana were characterized for their activity in whole body and different body segments (apical, intermediate, posterior), substrate affinity (acetyl-, butyryl-, propionylthiocholine), kinetic parameters (Km and Vmax) and in vitro response to model inhibitors (eserine hemisulfate, isoOMPA, BW284C51) and carbamates (carbofuran, methomyl, aldicarb and carbaryl). Results showed that the rate of hydrolysis for acetyl- and propionylthiocholine was higher in the posterior segment than the apical/intermediate segments and whole body. Cholinesterases of D. neapolitana showed a substrate preference for acetylthiocholine followed by propionylthiocholine; butyrylthioline was poorly hydrolyzed indicating, together with the absence of inhibition by the specific inhibitor and the absence of reactive bands in native electrophoresis, a lack of an active butyrylcholinesterase, differently than that observed in other Annelida species. The degree of inhibition by selected carbamates of cholinesterase activity with propionylthiocholine as substrate was higher than that observed with ATChI-ChE activity; aldicarb showed the highest inhibitory effect.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaguicidas / Poliquetos / Contaminantes Químicos del Agua / Carbamatos / Monitoreo del Ambiente / Inhibidores de la Colinesterasa / Colinesterasas Límite: Animals Idioma: En Revista: Comp Biochem Physiol C Toxicol Pharmacol Asunto de la revista: FARMACOLOGIA / TOXICOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaguicidas / Poliquetos / Contaminantes Químicos del Agua / Carbamatos / Monitoreo del Ambiente / Inhibidores de la Colinesterasa / Colinesterasas Límite: Animals Idioma: En Revista: Comp Biochem Physiol C Toxicol Pharmacol Asunto de la revista: FARMACOLOGIA / TOXICOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos