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Isolation of a simian immunodeficiency virus from a malbrouck (Chlorocebus cynosuros).
Carr, Michael; Kawaguchi, Akira; Sasaki, Michihito; Gonzalez, Gabriel; Ito, Kimihito; Thomas, Yuka; Hang'ombe, Bernard M; Mweene, Aaron S; Zhao, Guoyan; Wang, David; Orba, Yasuko; Ishii, Akihiro; Sawa, Hirofumi.
Afiliación
  • Carr M; Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan.
  • Kawaguchi A; National Virus Reference Laboratory, University College Dublin, Belfield, Dublin 4, Ireland.
  • Sasaki M; Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan.
  • Gonzalez G; Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan.
  • Ito K; Division of Bioinformatics, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan.
  • Thomas Y; Division of Bioinformatics, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan.
  • Hang'ombe BM; Hokudai Center for Zoonosis Control in Zambia, Research Center for Zoonosis Control, Hokkaido University, Lusaka, Zambia.
  • Mweene AS; Department of Para-clinical studies, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.
  • Zhao G; Department of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.
  • Wang D; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
  • Orba Y; Molecular Microbiology and Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
  • Ishii A; Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan.
  • Sawa H; Hokudai Center for Zoonosis Control in Zambia, Research Center for Zoonosis Control, Hokkaido University, Lusaka, Zambia.
Arch Virol ; 162(2): 543-548, 2017 Feb.
Article en En | MEDLINE | ID: mdl-27804019
ABSTRACT
To investigate the diversity of simian immunodeficiency virus (SIV) among nonhuman primates (NHPs) in Zambia, next-generation sequencing was performed to determine the complete genome sequence of a novel SIV recovered by co-culturing African green monkey (AGM) peripheral blood lymphocytes with human CD4+ T-cell lines. We report the first described SIV (SIVagmMAL-ZMB) from a malbrouck (Chlorocebus cynosuros). SIVagmMAL-ZMB was detected by real-time PCR analysis of splenic RNA in 3.2% (3/94) of AGMs and was undetectable in baboons (0/105). SIVagmMAL-ZMB possessed <80% nucleotide sequence identity to known SIV isolates and was located basally to vervet monkey SIV strains in all phylogenies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / ARN Viral / Síndrome de Inmunodeficiencia Adquirida del Simio / Virus de la Inmunodeficiencia de los Simios / Cercopithecinae Límite: Animals / Humans País/Región como asunto: Africa Idioma: En Revista: Arch Virol Año: 2017 Tipo del documento: Article País de afiliación: Japón
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / ARN Viral / Síndrome de Inmunodeficiencia Adquirida del Simio / Virus de la Inmunodeficiencia de los Simios / Cercopithecinae Límite: Animals / Humans País/Región como asunto: Africa Idioma: En Revista: Arch Virol Año: 2017 Tipo del documento: Article País de afiliación: Japón