Metabolic engineering of Saccharomyces cerevisiae for de novo production of dihydrochalcones with known antioxidant, antidiabetic, and sweet tasting properties.
Metab Eng
; 39: 80-89, 2017 01.
Article
en En
| MEDLINE
| ID: mdl-27810393
ABSTRACT
Dihydrochalcones are plant secondary metabolites comprising molecules of significant commercial interest as antioxidants, antidiabetics, or sweeteners. To date, their heterologous biosynthesis in microorganisms has been achieved only by precursor feeding or as minor by-products in strains engineered for flavonoid production. Here, the native ScTSC13 was overexpressed in Saccharomyces cerevisiae to increase its side activity in reducing p-coumaroyl-CoA to p-dihydrocoumaroyl-CoA. De novo production of phloretin, the first committed dihydrochalcone, was achieved by co-expression of additional relevant pathway enzymes. Naringenin, a major by-product of the initial pathway, was practically eliminated by using a chalcone synthase from barley with unexpected substrate specificity. By further extension of the pathway from phloretin with decorating enzymes with known specificities for dihydrochalcones, and by exploiting substrate flexibility of enzymes involved in flavonoid biosynthesis, de novo production of the antioxidant molecule nothofagin, the antidiabetic molecule phlorizin, the sweet molecule naringin dihydrochalcone, and 3-hydroxyphloretin was achieved.
Palabras clave
3-Hydroxyphloretin (PubChem CID: 11778945); Dihydrochalcone; Double bond reductase; Naringenin (PubChem CID: 932); Naringin dihydrochalcone; Naringin dihydrochalcone (PubChem CID: 9894584); Nothofagin; Nothofagin (PubChem CID: 42607691); Phloretin (PubChem CID: 4788); Phlorizin; Phlorizin (PubChem CID: 6072); Pinocembrin (PubChem CID: 68071); Saccharomyces cerevisiae; Trilobatin (PubChem CID: 6451798)
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
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Edulcorantes
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Proteínas de Saccharomyces cerevisiae
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Chalconas
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Ingeniería Metabólica
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Hipoglucemiantes
Idioma:
En
Revista:
Metab Eng
Asunto de la revista:
ENGENHARIA BIOMEDICA
/
METABOLISMO
Año:
2017
Tipo del documento:
Article
País de afiliación:
Alemania