A conserved inhibitory role of suppressor of cytokine signaling 1 (SOCS1) in salmon antiviral immunity.
Dev Comp Immunol
; 67: 66-76, 2017 02.
Article
en En
| MEDLINE
| ID: mdl-27818171
ABSTRACT
The SOCS proteins are regulators of JAK/STAT signaling. A number of viral infections has been associated with SOCS upregulation. Here we report that SOCS1 mRNA expression is up-regulated in salmon alphavirus (SAV3) infected TO cells, while infectious pancreatic necrosis virus infection has a negligible effect. SAV3 infected salmon showed increased SOCS1 mRNA levels in heart correlating with increased viral transcripts. Together, the in vitro and in vivo data suggests that SAV3-induced SOCS1 expression may affect the outcome of the virus infection. Using CHSE-214 cells overexpressing SOCS1 we revealed increased SAV3 replication compared to control, suggesting that SOCS1 suppress the antiviral capacity of the cells. In IFNa1-treated cells, the suppression of viral replication was partially rescued by SOCS1 overexpression. The increased viral replication in SOCS1 transgene cells was likely a result of impaired IFN-signaling and the reduced expression of interferon-stimulated genes in the transgene cells underscores this.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Salmón
/
Virus de la Necrosis Pancreática Infecciosa
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Infecciones por Birnaviridae
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Infecciones por Alphavirus
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Alphavirus
/
Proteínas de Peces
/
Enfermedades de los Peces
/
Proteína 1 Supresora de la Señalización de Citocinas
Límite:
Animals
Idioma:
En
Revista:
Dev Comp Immunol
Año:
2017
Tipo del documento:
Article
País de afiliación:
Noruega