Real-time monitoring of vesicle pH in an endocytic pathway using an EGF-conjugated two-photon probe.

Heo, Cheol Ho; Cho, Myoung Ki; Shin, Seunggun; Yoo, Tae Hyeon; Kim, Hwan Myung

Heo, Cheol Ho; Cho, Myoung Ki; Shin, Seunggun; Yoo, Tae Hyeon; Kim, Hwan Myung.

Afiliación

Heo CH; Department of Energy Systems Research, Ajou University, Suwon 443-749, Korea. kimhm@ajou.ac.kr.
Cho MK; Department of Energy Systems Research, Ajou University, Suwon 443-749, Korea. kimhm@ajou.ac.kr.
Shin S; Department of Molecular Science and Technology, Ajou University, Suwon 433-721, Korea. taehyeonyoo@ajou.ac.kr.
Yoo TH; Department of Molecular Science and Technology, Ajou University, Suwon 433-721, Korea. taehyeonyoo@ajou.ac.kr.
Kim HM; Department of Energy Systems Research, Ajou University, Suwon 443-749, Korea. kimhm@ajou.ac.kr.

Chem Commun (Camb) ; 52(97): 14007-14010, 2016 Nov 29.

Article en En | MEDLINE | ID: mdl-27853757

RESUMEN

Herein, we developed a ratiometric two-photon probe (BHS3-EGF), derived from a pH sensitive dye and epidermal growth factor (EGF), for real-time monitoring and quantitative analysis of acidic luminal pH values during endocytic pathway activity. Two-photon microscopy imaging with BHS3-EGF allows the quantitative analysis of pH distributions of single vesicles and their dynamics in receptor-mediated endocytosis in real-time.

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Chem Commun (Camb) Asunto de la revista: QUIMICA Año: 2016 Tipo del documento: Article Pais de publicación: Reino Unido

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links