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SRB1 as a new redox target of cigarette smoke in human sebocytes.
Crivellari, Ilaria; Sticozzi, Claudia; Belmonte, Giuseppe; Muresan, Ximena M; Cervellati, Franco; Pecorelli, Alessandra; Cavicchio, Carlotta; Maioli, Emanuela; Zouboulis, Christos C; Benedusi, Mascia; Cervellati, Carlo; Valacchi, Giuseppe.
Afiliación
  • Crivellari I; Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy; Department of Biomedical and Specialist Surgical Sciences, Section of Medical Biochemistry, Molecular Biology and Genetics, University of Ferrara, Ferrara, Italy.
  • Sticozzi C; Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
  • Belmonte G; Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
  • Muresan XM; Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
  • Cervellati F; Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
  • Pecorelli A; Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
  • Cavicchio C; Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
  • Maioli E; Department of Life Sciences, University of Siena, Siena, Italy.
  • Zouboulis CC; Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Dessau, Germany.
  • Benedusi M; Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
  • Cervellati C; Department of Biomedical and Specialist Surgical Sciences, Section of Medical Biochemistry, Molecular Biology and Genetics, University of Ferrara, Ferrara, Italy.
  • Valacchi G; Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy; Department of Animal Science, Plants for Human Health Institute, North Caroline State University, NC Research Campus, Kannapolis, NC, USA. Electronic address: giuseppe.valacchi@unife.it.
Free Radic Biol Med ; 102: 47-56, 2017 01.
Article en En | MEDLINE | ID: mdl-27865981
For its critical location, the skin represents the major interface between the body and the environment, therefore is one of the major biological barriers against the outdoor environmental stressors. Among the several oxidative environmental stressors, cigarette smoke (CS) has been associated with the development and worsening of many skin pathologies such as acne, dermatitis, delayed wound healing, aging and skin cancer. In our previous work we have demonstrated that CS is able to affect genes involved in skin cholesterol trafficking, among which SRB1, a receptor involved in the uptake of cholesterol from HDL, seems to be very susceptible to the oxidative stress induced by CS. In the present work we wanted to investigate the presence of SRB1 in human sebocytes and whether CS can affect cholesterol cellular uptake via the redox modulation of SRB1. By using a co-culture system of keratinocytes/sebocytes, we found that CS exposure induced a SRB1 protein loss without affecting sebocytes viability. The decrease of SRB1 levels was a consequence of SRB1/HNE adducts formation that leads to SRB1 ubiquitination and degradation. Moreover, the CS-induced loss of SRB1 induced an alteration of sebocytes lipid content, also demonstrated by cholesterol quantification in SRB1 siRNA experiments. In conclusion, exposure to CS, induced SRB1 post-translational modifications in sebocytes and this might affect sebocytes/skin functionality.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Queratinocitos / Estrés Oxidativo / Receptores Depuradores de Clase B / Fumar Cigarrillos Límite: Humans Idioma: En Revista: Free Radic Biol Med Asunto de la revista: BIOQUIMICA / MEDICINA Año: 2017 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Queratinocitos / Estrés Oxidativo / Receptores Depuradores de Clase B / Fumar Cigarrillos Límite: Humans Idioma: En Revista: Free Radic Biol Med Asunto de la revista: BIOQUIMICA / MEDICINA Año: 2017 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos