Photometry unlocks 3D information from 2D localization microscopy data.
Nat Methods
; 14(1): 41-44, 2017 01.
Article
en En
| MEDLINE
| ID: mdl-27869814
ABSTRACT
We developed a straightforward photometric method, temporal, radial-aperture-based intensity estimation (TRABI), that allows users to extract 3D information from existing 2D localization microscopy data. TRABI uses the accurate determination of photon numbers in different regions of the emission pattern of single emitters to generate a z-dependent photometric parameter. This method can determine fluorophore positions up to 600 nm from the focal plane and can be combined with biplane detection to further improve axial localization.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fotometría
/
Albúmina Sérica Bovina
/
Proteínas de Unión al GTP rab3
/
Imagenología Tridimensional
/
Imagen Molecular
/
Microscopía
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2017
Tipo del documento:
Article
País de afiliación:
Alemania