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Generation of camelid VHH bispecific constructs via in-cell intein-mediated protein trans-splicing.
Shibuya, Yuki; Haga, Natsuki; Asano, Ryutaro; Nakazawa, Hikaru; Hattori, Takamitsu; Takeda, Daisuke; Sugiyama, Aruto; Kurotani, Reiko; Kumagai, Izumi; Umetsu, Mitsuo; Makabe, Koki.
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  • Shibuya Y; Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jyonan, Yonezawa, Yamagata 992-8510, Japan.
  • Haga N; Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jyonan, Yonezawa, Yamagata 992-8510, Japan.
  • Asano R; Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 183-8538, Japan.
  • Nakazawa H; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, 6-6-11-606 Aoba-yama, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
  • Hattori T; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, 6-6-11-606 Aoba-yama, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
  • Takeda D; Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jyonan, Yonezawa, Yamagata 992-8510, Japan.
  • Sugiyama A; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, 6-6-11-606 Aoba-yama, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
  • Kurotani R; Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jyonan, Yonezawa, Yamagata 992-8510, Japan.
  • Kumagai I; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, 6-6-11-606 Aoba-yama, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
  • Umetsu M; Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, 6-6-11-606 Aoba-yama, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
  • Makabe K; Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jyonan, Yonezawa, Yamagata 992-8510, Japan makabe@yz.yamagata-u.ac.jp.
Protein Eng Des Sel ; 30(1): 15-21, 2017 Jan.
Article en En | MEDLINE | ID: mdl-27881685
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ingeniería de Proteínas / Anticuerpos Biespecíficos / Trans-Empalme / Inteínas / Anticuerpos de Dominio Único Límite: Animals / Humans Idioma: En Revista: Protein Eng Des Sel Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Buscar en Google
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PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ingeniería de Proteínas / Anticuerpos Biespecíficos / Trans-Empalme / Inteínas / Anticuerpos de Dominio Único Límite: Animals / Humans Idioma: En Revista: Protein Eng Des Sel Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido