Ezrin and moesin expression in canine and feline osteosarcoma.
Histol Histopathol
; 32(8): 805-816, 2017 Aug.
Article
en En
| MEDLINE
| ID: mdl-27900754
ABSTRACT
Biological features of canine osteosarcomas (OS) differ markedly from those found in feline and resemble more human osteosarcomas, in particular for their high rate of metastasis and poor prognosis. Ezrin, radixin and moesin are members of the ERM protein family and link the actin cytoskeleton with the cell membrane. Ezrin and moesin have been shown to be of prognostic significance in tumor progression due to their role in the metastatic process. The objective of this study was to analyze ezrin and moesin protein expression in a series of dog (n = 16) and cat (n = 8) osteosarcoma samples using immunohistochemistry and western blot techniques. We found that cat OS have a higher moesin expression compared to dog OS, however, the active phosphorylated forms of moesin and ezrin Tyr353 were more abundant in the dog samples. A statistically significant difference was found for the low and high immunohistochemical scores of ezrin and pan-phospho-ERM proteins between cat and dog. Although phospho-ezrin Thr567 was higher in feline OS, the membranous localization in dog OS samples indicates the presence of the biologically active form. Therefore, the observed differences in phosphorylated forms of ezrin and moesin status should be further studied to demonstrate if they are relevant for different biological behavior between dog and cat OS.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Óseas
/
Osteosarcoma
/
Regulación Neoplásica de la Expresión Génica
/
Proteínas del Citoesqueleto
/
Proteínas de Microfilamentos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Histol Histopathol
Asunto de la revista:
HISTOLOGIA
/
PATOLOGIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Austria