Neural Differentiation of Mesenchymal Stem Cells on Scaffolds for Nerve Tissue Engineering Applications.
Cell Reprogram
; 18(6): 369-381, 2016 11.
Article
en En
| MEDLINE
| ID: mdl-27906586
ABSTRACT
Scaffolds produced by electrospinning act as supports for cell proliferation and differentiation, improved through the release of neurotrophic factors. The objective of this study was to develop aligned and random nanofiber scaffolds with and without nerve growth factor to evaluate the potential of mesenchymal stem cells (MSCs) for neural differentiation. Nanofiber morphology, diameter, degradability, cell morphology, adhesion, proliferation, viability, cytotoxicity, and neural differentiation were performed to characterize the scaffolds. The expression for nestin, ß-III tubulin, and neuron-specific enolase was also evaluated. The scaffolds demonstrated a satisfactory environment for MSC growth, being nontoxic. The MSCs cultivated on the scaffolds were able to adhere and proliferate. The evaluation of neural differentiation indicated that in all groups of scaffolds the MSCs were able to upregulate neural gene expression.
Palabras clave
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Madre
/
Ingeniería de Tejidos
/
Pulpa Dental
/
Andamios del Tejido
/
Neurogénesis
/
Células Madre Mesenquimatosas
/
Neuronas
Límite:
Humans
Idioma:
En
Revista:
Cell Reprogram
Año:
2016
Tipo del documento:
Article
País de afiliación:
Brasil