Nonlinear optical corneal collagen crosslinking of ex vivo rabbit eyes.
J Cataract Refract Surg
; 42(11): 1660-1665, 2016 11.
Article
en En
| MEDLINE
| ID: mdl-27956294
ABSTRACT
PURPOSE:
To determine whether riboflavin-induced collagen crosslinking (CXL) could be precisely achieved in the corneal stroma of ex vivo rabbit eyes using nonlinear optical excitation with a low numerical aperture lens and enlarged focal volume.SETTING:
Gavin Herbert Eye Institute, University of California Irvine, Irvine, California, USA.DESIGN:
Experimental study.METHODS:
The corneal epithelium was removed and the corneas were soaked in 0.5% riboflavin solution. Using a 0.1 numerical aperture objective, a theoretical excitation volume of 150 µm × 3 µm was generated using 1 W of 760 nm femtosecond laser light and raster scanned with 4.4 µm line separation at varying effective speeds over a 4.50 mm × 2.25 mm area. Corneal sections were examined for collagen autofluorescence.RESULTS:
Collagen autofluorescence was enhanced 2.9 times compared with ultraviolet-A (UVA) CXL. Also, increasing speed was linearly associated with decreasing autofluorescence intensity. The slowest speed of 2.69 mm/s showed a mean of 182.97 µm ± 52.35 (SD) long autofluorescent scan lines axially in the central cornea compared with 147.84 ± 4.35 µm for UVA CXL.CONCLUSIONS:
Decreasing dwell time was linearly associated with decreasing autofluorescence intensity, approaching that of UVA CXL at a speed of 8.9 mm/s. Using an effective speed of 8.9 mm/s, nonlinear optical CXL could be achieved over a 3.0 mm diameter area in fewer than 4 minutes. Further development of nonlinear optical CXL might result in safer, faster, and more effective CXL treatments. FINANCIAL DISCLOSURE None of the authors has a financial or proprietary interest in any material or method mentioned.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Colágeno
/
Córnea
/
Sustancia Propia
/
Reactivos de Enlaces Cruzados
Límite:
Animals
Idioma:
En
Revista:
J Cataract Refract Surg
Asunto de la revista:
OFTALMOLOGIA
Año:
2016
Tipo del documento:
Article
País de afiliación:
Estados Unidos