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Molecular Characterization of Animal Fasciola spp. Isolates from Kermanshah, Western Iran.
Bozorgomid, Arezoo; Nazari, Naser; Rahimi, Hamzeh; Beigom Kia, Eshrat; Hajjaran, Homa; Mohebali, Mehdi; Aryaeipour, Mojgan; Heidarian, Peyman; Ezati, Mohammad Saeid; Rokni, Mohamad Bagher.
Afiliación
  • Bozorgomid A; Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Nazari N; Dept. of Medical Parasitology and Mycology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
  • Rahimi H; Dept. of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
  • Beigom Kia E; Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Hajjaran H; Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Mohebali M; Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.
  • Aryaeipour M; Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Heidarian P; Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Ezati MS; Dept. of Food Hygiene, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
  • Rokni MB; Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.
Iran J Public Health ; 45(10): 1315-1321, 2016 Oct.
Article en En | MEDLINE | ID: mdl-27957438
ABSTRACT

BACKGROUND:

We evaluated the genetic diversity of samples identified morphologically as Fasciola spp. from sheep, cattle and goat from Kermanshah Province, western Iran using PCR-RFLP method.

METHODS:

We used PCR-RFLP analysis of ribosomal ITS1 fragment using RsaI restriction enzyme to investigate the genetic characteristics of Fasciola species obtained from different hosts (16 sheep, 28 cattle, 4 goats). The species of Fasciola were confirmed by sequencing the 700 bp region of ribosomal ITS1 gene.

RESULTS:

In Kermanshah, F. hepatica was present in 96% of the samples, F. gigantica was found only in two cattle sample. No hybrid forms were detected in the present study.

CONCLUSION:

Our results contribute to clarify the dark spots of Fasciola genotyping in different parts of Iran.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Iran J Public Health Año: 2016 Tipo del documento: Article País de afiliación: Irán
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Iran J Public Health Año: 2016 Tipo del documento: Article País de afiliación: Irán