A novel pretreatment method combining sealing technique with direct injection technique applied for improving biosafety.
Bioanalysis
; 9(2): 173-182, 2017 Jan.
Article
en En
| MEDLINE
| ID: mdl-27960549
ABSTRACT
AIM:
People today have a stronger interest in the risk of biosafety in clinical bioanalysis. A safe, simple, effective method of preparation is needed urgently. METHODOLOGY/RESULTS:
To improve biosafety of clinical analysis, we used antiviral drugs of adefovir and tenofovir as model drugs and developed a safe pretreatment method combining sealing technique with direct injection technique. The inter- and intraday precision (RSD %) of the method were <4%, and the extraction recoveries ranged from 99.4 to 100.7%. Meanwhile, the results showed that standard solution could be used to prepare calibration curve instead of spiking plasma, acquiring more accuracy result. CONCLUSION/DISCUSSION:
Compared with traditional methods, the novel method not only improved biosecurity of the pretreatment method significantly, but also achieved several advantages including higher precision, favorable sensitivity and satisfactory recovery. With these highly practical and desirable characteristics, the novel method may become a feasible platform in bioanalysis.Palabras clave
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Antivirales
/
Adenina
/
Técnicas de Laboratorio Clínico
/
Organofosfonatos
/
Tenofovir
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Bioanalysis
Año:
2017
Tipo del documento:
Article
País de afiliación:
China