Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia.

Zhao, Xi; Huang, Xiaomeng; Wang, Xinmei; Wu, Yun; Eisfeld, Ann-Kathrin; Schwind, Sebastian; Gallego-Perez, Daniel; Boukany, Pouyan E; Marcucci, Guido I; Lee, Ly James

Zhao, Xi; Huang, Xiaomeng; Wang, Xinmei; Wu, Yun; Eisfeld, Ann-Kathrin; Schwind, Sebastian; Gallego-Perez, Daniel; Boukany, Pouyan E; Marcucci, Guido I; Lee, Ly James.

Afiliación

Zhao X; Center for Affordable Nanoengineering of Polymeric Biomedical Devices The Ohio State University Columbus OH 43212 USA; William G. Lowrie, Department of Chemical and Biomolecular Engineering The Ohio State University Columbus OH 43210 USA.
Huang X; Center for Affordable Nanoengineering of Polymeric Biomedical Devices The Ohio State University Columbus OH 43212 USA.
Wang X; Center for Affordable Nanoengineering of Polymeric Biomedical Devices The Ohio State University Columbus OH 43212 USA.
Wu Y; Center for Affordable Nanoengineering of Polymeric Biomedical Devices The Ohio State University Columbus OH 43212 USA.
Eisfeld AK; Department of Internal Medicine Comprehensive Cancer Center The Ohio State University Columbus OH 43210 USA.
Schwind S; Department of Internal Medicine Comprehensive Cancer Center The Ohio State University Columbus OH 43210 USA.
Gallego-Perez D; Center for Affordable Nanoengineering of Polymeric Biomedical Devices The Ohio State University Columbus OH 43212 USA.
Boukany PE; Center for Affordable Nanoengineering of Polymeric Biomedical Devices The Ohio State University Columbus OH 43212 USA.
Marcucci GI; Department of Internal Medicine Comprehensive Cancer Center The Ohio State University Columbus OH 43210 USA.
Lee LJ; Center for Affordable Nanoengineering of Polymeric Biomedical Devices The Ohio State University Columbus OH 43212 USA; William G. Lowrie, Department of Chemical and Biomolecular Engineering The Ohio State University Columbus OH 43210 USA.

Adv Sci (Weinh) ; 2(12): 1500111, 2015 12.

Article en En | MEDLINE | ID: mdl-27980918

ABSTRACT

ABSTRACT

A living cell interrogation platform based on nanochannel electroporation is demonstrated with analysis of RNAs in single cells. This minimally invasive process is based on individual cells and allows both multi-target analysis and stimulus-response analysis by sequential deliveries. The unique platform possesses a great potential to the comprehensive and lysis-free nucleic acid analysis on rare or hard-to-transfect cells.

Palabras clave

RNA recognition; fluorescent probes; living cell interrogation; nanochannel electroporation

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Adv Sci (Weinh) Año: 2015 Tipo del documento: Article