A new approach for the immobilization of poly(acrylic) acid as a chemically reactive cross-linker on the surface of poly(lactic) acid-based biomaterials.
Mater Sci Eng C Mater Biol Appl
; 71: 862-869, 2017 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-27987783
ABSTRACT
A new approach for the immobilization of poly(acrylic) acid (PAA) as a chemically reactive cross-linker on the surface of poly(lactic) acid-based (PLA) biomaterials is described. The proposed technique includes non-covalent attachment of a PAA layer to the surface of PLA-based biomaterial via biomaterial surface treatment with solvent/non-solvent mixture followed by the entrapment of PAA from its solution. Surface morphology and wettability of the obtained PLA-PAA composite materials were investigated by AFM and the sitting drop method respectively. The amount of the carboxyl groups on the composites surface was determined by using the fluorescent compounds (2-(5-aminobenzo[d]oxazol-2-yl)phenol (ABO) and its acyl derivative N-(2-(2-hydroxyphenyl)benzo[d]oxazol-5-yl)acetamide (AcABO)). It was shown that it is possible to obtain PLA-PAA composites with various surface relief and tunable wettability (57°, 62° and 66°). The capacity of the created PAA layer could be varied from 1.5nmol/cm2 to 0.1µmol/cm2 depending on the modification conditions. Additionally, using bovine serum albumin (BSA) it was demonstrated that such composites could be modified with proteins with high binding density (around 0.18nmol/cm2). Obtained fluoro-labeled PLA-PAA materials, as well as PLA-PAA composites themselves, are valuable since they can be used for biodegradable polymer implants tracking in living systems and as drug delivery systems.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Poliésteres
/
Resinas Acrílicas
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Sistemas de Liberación de Medicamentos
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Plásticos Biodegradables
Límite:
Animals
Idioma:
En
Revista:
Mater Sci Eng C Mater Biol Appl
Año:
2017
Tipo del documento:
Article
País de afiliación:
Rusia