Outlook on optimizing ultramicroscopy imaging technique through optical characterization.
Microsc Res Tech
; 81(9): 929-935, 2018 Sep.
Article
en En
| MEDLINE
| ID: mdl-28000307
ABSTRACT
Here, we present an optically optimized system for static ultramicroscopy imaging technique. The unit for generating an ultra-thin light sheet employs aspheric and meso-optical elements (meso-aspheric system). An analytical as well as an experimental comparison between the light sheet produced by the standard system (using a rectangular slit aperture and one cylindrical lens) and the one produced by our latest optimized system, which converts a symmetrical Gaussian beam into an ultra-thin light sheet is presented. Using the new light sheet in combination with our objective equipped with a modulator unit to compensate the refractive index mismatch between air and mediums with indices of 1.45-1.56, we present high resolution images of various biological samples that were chemically cleared using different methods. They demonstrate a marked improvement in quality, contrast and resolution.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Microsc Res Tech
Asunto de la revista:
DIAGNOSTICO POR IMAGEM
Año:
2018
Tipo del documento:
Article
País de afiliación:
Austria