A microfluidic paper-based device to assess acetylcholinesterase activity.
Electrophoresis
; 38(7): 1002-1006, 2017 04.
Article
en En
| MEDLINE
| ID: mdl-28008633
ABSTRACT
Neurotransmitters play key roles in cell-to-cell communication. These chemical messengers are involved in many functional processes, including growth, reproduction, memory, and behavior. In this communication, we describe a novel microfluidic paper-based analytical device (µPAD) to detect acetylcholinesterase (AChE) activity and inhibitor screening through a colorimetric analysis. The µPAD is easily fabricated via a wax printing process whereby wax is deposited onto the surface of chromatographic paper, and heated to create a hydrophobic barrier. Separate solutions of 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) and samples containing AChE and acetylthiocholine iodide (ATC) (or cysteine, Cys), respectively, are directly spotted onto the µPAD. DTNB and AChE/ATC (or Cys) flow towards each other where a reaction occurs to form the yellow colored 2-nitro-5-thiobenzoic acid anion (TNB2- ). The device is dried, scanned, and analyzed yielding a linear range of average inverse yellow intensities versus substrate concentration. An IC50 value (0.045 nM) with a known inhibitor, neostigmine bromide (NB), is obtained on the device. µPADs are low cost and easy to fabricate and have great potential to quantify neurotransmitter activity.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Acetilcolinesterasa
/
Técnicas Analíticas Microfluídicas
Idioma:
En
Revista:
Electrophoresis
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos