Proteomic-based biomarker discovery for development of next generation diagnostics.
Appl Microbiol Biotechnol
; 101(2): 475-491, 2017 Jan.
Article
en En
| MEDLINE
| ID: mdl-28013407
ABSTRACT
In the post-genome age, proteomics is receiving significant attention because they provide an invaluable source of biological structures and functions at the protein level. The search for disease-specific biomarkers for diagnostic and/or therapeutic applications is one of the areas that proteomics is having a significant impact. Thus, the identification of a "good" biomarker enables a more accurate early diagnosis and prognosis of disease. Rapid advancements in mass spectrometry (MS) instrumentation, liquid chromatography MS (LCMS), protein microarray technology, and other protein profiling methodologies have a substantial expansion of our toolbox to identify disease-specific protein and peptide biomarkers. This review covers a selection of widely used proteomic technologies for biomarker discovery. In addition, we describe the most commonly used approaches for diagnosis based on proteomic biomarkers and further discuss trends and critical challenges during development of cost-effective rapid diagnostic tests and microfluidic diagnostic systems based on proteomic biomarkers.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biomarcadores
/
Proteómica
/
Pruebas Diagnósticas de Rutina
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos