Spectral Domain Optical Coherence Tomography: An In Vivo Imaging Protocol for Assessing Retinal Morphology in Adult Zebrafish.
Zebrafish
; 14(2): 118-125, 2017 04.
Article
en En
| MEDLINE
| ID: mdl-28051361
ABSTRACT
The present study outlines a protocol for examining retinal structure in zebrafish, a popular model organism for ocular studies, using spectral domain optical coherence tomography (SD-OCT). We demonstrate how this live imaging modality can be used to obtain high quality images of several retinal features, including the optic nerve, retinal vasculature, and the cone photoreceptor mosaic. Retinal histology sections were obtained from imaged fish for comparison with SD-OCT cross-sectional B-scans. Voronoi domain analysis was used to assess cone photoreceptor packing regularity at 3, 6, and 12 months. SD-OCT is an effective in vivo technique for studying the adult zebrafish retina and can be applied to disease models for longitudinal serial monitoring.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Fotorreceptoras
/
Pez Cebra
/
Tomografía de Coherencia Óptica
Límite:
Animals
Idioma:
En
Revista:
Zebrafish
Asunto de la revista:
BIOLOGIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Reino Unido