Co-culture with macrophages enhances the clonogenic and invasion activity of endometriotic stromal cells.
Cell Prolif
; 50(3)2017 Jun.
Article
en En
| MEDLINE
| ID: mdl-28071833
ABSTRACT
OBJECTIVE:
To study the effect on endometrial and endometriotic cells after co-culture with macrophages, using clonogenic, invasion and self-renewal assays. MATERIALS ANDMETHODS:
Peripheral blood samples, endometrium and endometriotic tissues were collected. Autologous macrophages were co-cultured with endometrial and endometriotic cells. The number of colony-forming units (CFU), invasiveness and self-renewal activity after co-culture with macrophages were determined. The cytokine level of colony-stimulating factor-1 (CSF-1) from macrophages with and without endometriosis was compared.RESULTS:
Co-culture with macrophages significantly increased the clonogenic and invasion ability of endometriotic stromal cells in vitro. Colony-stimulating factor-1 (CSF-1) was up-regulated in endometriotic macrophages conditioned medium when compared to those without the disease.CONCLUSIONS:
These data suggest that macrophages may increase the proliferation and invasion activity of stromal clonogenic cells in women with endometriosis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Movimiento Celular
/
Células Clonales
/
Células del Estroma
/
Técnicas de Cocultivo
/
Endometrio
/
Macrófagos
Límite:
Adult
/
Female
/
Humans
/
Middle aged
Idioma:
En
Revista:
Cell Prolif
Año:
2017
Tipo del documento:
Article
País de afiliación:
China